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Western blot analysis of extracts of various cell lines, using RPH3A antibody (15-087) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human liver cancer using RPH3A antibody (15-087) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using RPH3A antibody (15-087) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using RPH3A antibody (15-087) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using RPH3A antibody (15-087) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

RPH3A Antibody

CATALOG NUMBER: 15-087

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human RPH3A (NP_055769.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
Mouse brain
Positive Control 2:
Rat brain
Predicted Molecular Weight:
Observed: 77kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RPH3A
Additional Names:
Rabphilin-3A, Exophilin-1, RPH3A, KIAA0985
Ncbi Gene Id Number:
22895
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is thought to be an effector for RAB3A, which is a small G protein that acts in the late stages of neurotransmitter exocytosis. The encoded protein may be involved in neurotransmitter release and synaptic vesicle traffic.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-087

List Size:
100 uL

List Price:

$419.00

Out of stock

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