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Western Blot at 1:1000 dilution + A549 whole cell lysate Lysates/proteins at 20 ug per lane.
RPH3AL Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000 dilution + A549 whole cell lysate Lysates/proteins at 20 ug per lane.

RPH3AL Antibody

CATALOG NUMBER: 55-383

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This RPH3AL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 208-237 amino acids from the C-terminal region of human RPH3AL.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
34 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RPH3AL
Additional Names:
Rab effector Noc2, No C2 domains protein, Rabphilin-3A-like protein, RPH3AL, NOC2
Protein Accession Number:
Q9UNE2
Protein Gi Number:
74735140
Ncbi Gene Id Number:
9501
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene plays a direct regulatory role in calcium-ion-dependent exocytosis in both endocrine and exocrine cells and plays a key role in insulin secretion by pancreatic cells. This gene is likely a tumor suppressor. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Background Reference 1:
Rose, J. Phd, et al. Mol. Med. (2010) In pressShanmugam, C., et al. Biotech Histochem 84(2):39-45(2009) Katkoori, V.R., et al. Front. Biosci. 13, 1050-1061 (2008)Matsumoto, M., et al. Proc. Natl. Acad. Sci. U.S.A. 101(22):8313-8318(2004) Manabe, S., et al. Biochem. Biophys. Res. Commun. 316(1):218-225(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-383

List Size:
400 ul

List Price:

$495.00

Datasheet
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