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Western blot analysis of extracts of various cell lines, using RPIA antibody (23-747) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat heart using RPIA antibody (23-747) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using RPIA antibody (23-747) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using RPIA antibody (23-747) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.

RPIA Antibody

CATALOG NUMBER: 23-747

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 82-311 of human RPIA (NP_653164.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:200 - 1:1000
IHC: 1:50 - 1:200
Positive Control 1:
B cells
Positive Control 2:
HL-60
Positive Control 3:
Mouse thymus
Positive Control 4:
Rat fat
Positive Control 5:
Mouse spleen
Positive Control 6:
Mouse skeletal muscle
Predicted Molecular Weight:
Observed: 33kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RPIA
Additional Names:
RPIA, RPI
Ncbi Gene Id Number:
22934
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is an enzyme, which catalyzes the reversible conversion between ribose-5-phosphate and ribulose-5-phosphate in the pentose-phosphate pathway. This gene is highly conserved in most organisms. The enzyme plays an essential role in the carbohydrate metabolism. Mutations in this gene cause ribose 5-phosphate isomerase deficiency. A pseudogene is found on chromosome 18.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-747

List Size:
100 uL

List Price:

$436.00

Datasheet
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