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Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
RPL15 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

RPL15 Antibody

CATALOG NUMBER: 56-636

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Rat
Immunogen:
This RPL15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human RPL15.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
24 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RPL15
Additional Names:
60S ribosomal protein L15, RPL15, EC45
Protein Accession Number:
P61313
Protein Gi Number:
47117726
Ncbi Gene Id Number:
6138
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Ribosomes, the organelles that catalyze protein synthesis,consist of a small 40S subunit and a large 60S subunit. Togetherthese subunits are composed of 4 RNA species and approximately 80structurally distinct proteins. This gene encodes a ribosomalprotein that is a component of the 60S subunit. The protein belongsto the L15E family of ribosomal proteins. It is located in thecytoplasm. This gene shares sequence similarity with the yeastribosomal protein YL10 gene. Although this gene has been referredto as RPL10, its official symbol is RPL15. This gene has been shownto be overexpressed in some esophageal tumors compared to normalmatched tissues. Transcript variants utilizing alternative polyAsignals exist. As is typical for genes encoding ribosomal proteins,there are multiple processed pseudogenes of this gene dispersedthrough the genome.
Background Reference 1:
Wheeler, H.E., et al. PLoS Genet.(10), E1000685 (2009) :
Background Reference 2:
Wang, H., et al. BMC Cancer 6, 91 (2006) :
Background Reference 3:
Andersen, J.S., et al. Nature 433(7021):77-83(2005)
Background Reference 4:
Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-636

List Size:
400 ul

List Price:

$495.00

Datasheet
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