Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis in K562 cell line lysates (35ug/lane).
Western blot analysis in CHO cell line lysates (35ug/lane).
RPL27A Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562 cell line lysates (35ug/lane).

RPL27A Antibody

CATALOG NUMBER: 55-518

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Hamster, Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Hamster, Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse, Rat
Immunogen:
This RPL27A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-138 amino acids from the C-terminal region of human RPL27A.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
17 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RPL27A
Additional Names:
60S ribosomal protein L27a, RPL27A
Protein Accession Number:
P46776
Ncbi Gene Id Number:
6157
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Ribosomes, the organelles that catalyze protein synthesis,consist of a small 40S subunit and a large 60S subunit. Togetherthese subunits are composed of 4 RNA species and approximately 80structurally distinct proteins. This gene encodes a ribosomalprotein that is a component of the 60S subunit. The protein belongsto the L15P family of ribosomal proteins. It is located in thecytoplasm. Variable expression of this gene in colorectal cancerscompared to adjacent normal tissues has been observed, although nocorrelation between the level of expression and the severity of thedisease has been found. As is typical for genes encoding ribosomalproteins, multiple processed pseudogenes derived from this gene aredispersed through the genome.
Background Reference 1:
Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004) :
Background Reference 2:
Mazumder, B., et al. Cell 115(2):187-198(2003)
Background Reference 3:
Andersen, J.S., et al. Curr. Biol. 12(1):1-11(2002)
Background Reference 4:
Bortoluzzi, S., et al. Bioinformatics 17(12):1152-1157(2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-518

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369