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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
RPL3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).

RPL3 Antibody

CATALOG NUMBER: 57-031

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse, Pig, Rat, Yeast
Immunogen:
This RPL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the Central region of human RPL3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
46 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RPL3
Additional Names:
60S ribosomal protein L3, HIV-1 TAR RNA-binding protein B, TARBP-B, RPL3
Protein Accession Number:
P39023
Protein Gi Number:
730565
Ncbi Gene Id Number:
6122
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Ribosomes, the complexes that catalyze protein synthesis,consist of a small 40S subunit and a large 60S subunit. Togetherthese subunits are composed of 4 RNA species and approximately 80structurally distinct proteins. This gene encodes a ribosomalprotein that is a component of the 60S subunit. The protein belongsto the L3P family of ribosomal proteins and it is located in thecytoplasm. The protein can bind to the HIV-1 TAR mRNA, and it hasbeen suggested that the protein contributes to tat-mediatedtransactivation. This gene is co-transcribed with several smallnucleolar RNA genes, which are located in several of this gene'sintrons. Alternate transcriptional splice variants, encodingdifferent isoforms, have been characterized. As is typical forgenes encoding ribosomal proteins, there are multiple processedpseudogenes of this gene dispersed through the genome. [provided byRefSeq].
Background Reference 1:
Russo, A., et al. Biochim. Biophys. Acta 1799 (5-6), 419-428 (2010) :
Background Reference 2:
Rikova, K., et al. Cell 131(6):1190-1203(2007)
Background Reference 3:
Tu, L.C., et al. Mol. Cell Proteomics 6(4):575-588(2007)
Background Reference 4:
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-031

List Size:
400 ul

List Price:

$495.00

Datasheet
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