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Western Blot at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: U-251 MG whole cell lysate Lane 4: U-2OS whole cell lysate Lane 5: mouse brain lysate Lane 6: NIH3T3 whole cell lysate Lane 7: rat liver lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in A375 cell line lysates (35ug/lane).
RPL34 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: U-251 MG whole cell lysate Lane 4: U-2OS whole cell lysate Lane 5: mouse brain lysate Lane 6: NIH3T3 whole cell lysate Lane 7: rat liver lysate Lysates/proteins at 20 ug per lane.

RPL34 Antibody

CATALOG NUMBER: 56-921

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Pig
Immunogen:
This RPL34 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-66 amino acids from the Central region of human RPL34.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
13 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RPL34
Additional Names:
60S ribosomal protein L34, RPL34
Protein Accession Number:
P49207
Ncbi Gene Id Number:
6164
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Ribosomes, the organelles that catalyze protein synthesis,consist of a small 40S subunit and a large 60S subunit. Togetherthese subunits are composed of 4 RNA species and approximately 80structurally distinct proteins. This gene encodes a ribosomalprotein that is a component of the 60S subunit. The protein belongsto the L34E family of ribosomal proteins. It is located in thecytoplasm. This gene originally was thought to be located at 17q21,but it has been mapped to 4q. Transcript variants derived fromalternative splicing, alternative transcription initiation sites,and/or alternative polyadenylation exist; these variants encode thesame protein. As is typical for genes encoding ribosomal proteins,there are multiple processed pseudogenes of this gene dispersedthrough the genome.
Background Reference 1:
Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Background Reference 2:
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Background Reference 3:
Andersen, J.S., et al. Nature 433(7021):77-83(2005)
Background Reference 4:
Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-921

List Size:
400 ul

List Price:

$519.00

Datasheet
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