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<strong>Figure 1 WB Validation of rpmB/L28 in E. coli </strong><br>Loading: 15 μg of lysate Antibodies: rpmB/L28 10059, 0.5 μg/mL , 1 h incubation at RT in 15% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 1 WB Validation of rpmB/L28 in E. coli
Loading: 15 μg of lysate Antibodies: rpmB/L28 10059, 0.5 μg/mL , 1 h incubation at RT in 15% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
New
Trial Size

rpmB/L28 Antibody

CATALOG NUMBER: 10059

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
E. coli
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
E. coli
Homology:
Predicted species reactivity based on immunogen sequence: Klebsiella pneumoniae: (100%), Escherichia fergusonii: (100%), Shigella: (100%)
Immunogen:
Anti-rpmB/L28 antibody (10059) was raised against an 19 amino acid peptide near the carboxy terminus of E. coli rpmB/L28.

The immunogen is located within the last 50 amino acids of rpmB/L28.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB: 0.5 μg/mL.

Antibody validated: Western Blot in E.coli samples. All other applications and species not yet tested.
Predicted Molecular Weight:
Predicted: 9 kD

Observed: 9 kD
Purification:
rpmB/L28 Antibody is affinity chromatography.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
rpmB/L28 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
rpmB/L28 Antibody can be stored at 4 ° C for three months and -20 ° C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
rpmB
Additional Names:
Large ribosomal subunit protein bL28, 50S ribosomal protein L28, rpmB
Protein Accession Number:
P0A7M2.2
Protein Gi Number:
67472014
Ncbi Gene Id Number:
946941
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The rpmB/L28 protein is a component of the 50S subunit of the ribosome and is required for ribosome assembly. rpmB/L28 interacts with 23S rRNA and crosslinks to L9. L15 and L17 stimulate the interactions of rpmB/L28 with 23S rRNA. rpmB/L28 is phosphorylated at Thr8. rpmB/L28 is labeled by the macrolides carbomycin A, niddamycin and tylosin, which inhibit ribosomal activity.
Background Reference 1:
Maguire, B A, and D G Wild. Molecular Microbiology. 1997, 23(2): 237-45.
Background Reference 2:
Sköld, S E. Biochimie 1981, 63(1): 53-60.
Background Reference 3:
Walleczek, J et al. The Journal of biological chemistry 1989, 264(7): 4231-7.
Background Reference 4:
Herold, M, and K H Nierhaus. The Journal of biological chemistry 1987, 262(18): 8826-33.
Background Reference 5:
Soung, George Y et al. Journal of proteome research 2009, 8(7): 3390-402.
Background Reference 6:
Tejedor, F, and J P Ballesta. Biochemistry 1985, 24 (2): 467-72.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

10059

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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