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<strong>Figure 1 WB Validation of rpmB/L28 in E. coli </strong><br>Loading: 15 μg of lysate Antibodies: rpmB/L28 10061, 0.5 μg/mL , 1 h incubation at RT in 15% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 1 WB Validation of rpmB/L28 in E. coli
Loading: 15 μg of lysate Antibodies: rpmB/L28 10061, 0.5 μg/mL , 1 h incubation at RT in 15% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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rpmB/L28 Antibody

CATALOG NUMBER: 10061

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
E. coli
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
E. coli
Homology:
Predicted species reactivity based on immunogen sequence: Vibrio: (100%), Salmonella: (100%), Klebsiella: (100%)
Immunogen:
Anti-rpmB/L28 antibody (10061) was raised against an 15 amino acid peptide near the amino terminus of E. coli rpmB/L28.

The immunogen is located within the first 50 amino acids of rpmB/L28.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB: 0.5 μg/mL.

Antibody validated: Western Blot in E.coli samples. All other applications and species not yet tested.
Predicted Molecular Weight:
Predicted: 9 kD

Observed: 9 kD
Purification:
rpmB/L28 Antibody is affinity chromatography.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
rpmB/L28 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
rpmB/L28 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
rpmB
Additional Names:
Large ribosomal subunit protein bL28, 50S ribosomal protein L28, rpmB
Protein Accession Number:
P0A7M2.2
Protein Gi Number:
67472014
Ncbi Gene Id Number:
946941
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The rpmB/L28 protein is a component of the 50S subunit of the ribosome and is required for ribosome assembly. rpmB/L28 interacts with 23S rRNA and crosslinks to L9. L15 and L17 stimulate the interactions of rpmB/L28 with 23S rRNA. rpmB/L28 is phosphorylated at Thr8. rpmB/L28 is labeled by the macrolides carbomycin A, niddamycin and tylosin, which inhibit ribosomal activity.
Background Reference 1:
Maguire, B A, and D G Wild. Molecular Microbiology. 1997, 23(2): 237-45.
Background Reference 2:
Sköld, S E. Biochimie 1981, 63(1): 53-60.
Background Reference 3:
Walleczek, J et al. The Journal of biological chemistry 1989, 264(7): 4231-7.
Background Reference 4:
Herold, M, and K H Nierhaus. The Journal of biological chemistry 1987, 262(18): 8826-33.
Background Reference 5:
Soung, George Y et al. Journal of proteome research 2009, 8(7): 3390-402.
Background Reference 6:
Tejedor, F, and J P Ballesta. Biochemistry 1985, 24 (2): 467-72.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

10061

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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