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<strong>Figure 1 WB Validation of rpmH/L34 in E. coli </strong><br>Loading: 15 μg of lysate Antibodies: rpmH/L34 10063, 0.5 μg/mL , 1 h incubation at RT in 15% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 1 WB Validation of rpmH/L34 in E. coli
Loading: 15 μg of lysate Antibodies: rpmH/L34 10063, 0.5 μg/mL , 1 h incubation at RT in 15% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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rpmH/L34 Antibody

CATALOG NUMBER: 10063

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
E. coli
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
E. coli
Homology:
Predicted species reactivity based on immunogen sequence: Yersinia: (100%), Shigella: (100%), Salmonella: (100%)
Immunogen:
Anti-rpmH/L34 antibody (10063) was raised against an 17 amino acid peptide near the amino terminus of E. coli rpmH/L34.

The immunogen is located within the first 30 amino acids of rpmH/L34.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB: 0.5 μg/mL.

Antibody validated: Western Blot in E.coli samples. All other applications and species not yet tested.
Predicted Molecular Weight:
Predicted: 5.4 kD

Observed: 6 kD
Purification:
rpmH/L34 Antibody is affinity chromatography.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
rpmH/L34 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
rpmH/L34 Antibody can be stored at 4 ° C for three months and -20 ° C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
rpmH
Additional Names:
rimA, ssaF, Large ribosomal subunit protein bL34, 50S ribosomal protein L34
Protein Accession Number:
P0A7P5.1
Protein Gi Number:
67472334
Ncbi Gene Id Number:
948216
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The rpmH/L34 protein is a component of the 50S subunit of the ribosome. rpmH/L34 can be isolated in a complex with 5S rRNA, tRNA, and other proteins of the large ribosomal subunit. rpmH/L34 is one of six proteins that is completely lost from ribosomes in post-stationary cells. rpmH/L34 was identified as “antizyme 2”, an inhibitor of the biosynthetic ornithine and arginine decarboxylases, enzymes are involved in the biosynthesis of polyamines.
Background Reference 1:
Matspalu, E et al. European journal of biochemistry 1982, 124(2): 269-73.
Background Reference 2:
Ramagopal, S. European journal of biochemistry 1984, 140(2): 353-61.
Background Reference 3:
Heller, J S et al. Proceedings of the National Academy of Sciences of the United States of America 1983, 80(17): 5181-4.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

10063

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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