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Western blot analysis in WiDr cell line lysates (35ug/lane).
Western blot analysis in WiDr cell line lysates (35ug/lane).

RPN1 Antibody

CATALOG NUMBER: 58-863

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey, Pig, Rat
Immunogen:
This RPN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 559-587 amino acids from the C-terminal region of human RPN1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
69 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RPN1
Additional Names:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, Ribophorin I, RPN-I, Ribophorin-1, RPN1
Protein Accession Number:
P04843
Protein Gi Number:
132559
Ncbi Gene Id Number:
6184
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a type I integral membrane protein foundonly in the rough endoplasmic reticulum. The encoded protein ispart of an N-oligosaccharyl transferase complex that links highmannose oligosaccharides to asparagine residues found in theAsn-X-Ser/Thr consensus motif of nascent polypeptide chains. Thisprotein forms part of the regulatory subunit of the 26S proteasomeand may mediate binding of ubiquitin-like domains to thisproteasome.
Background Reference 1:
Ruiz-Canada, C., et al. Cell 136(2):272-283(2009)
Background Reference 2:
Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008)
Background Reference 3:
Wilson, C.M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(28):9534-9539(2008)
Background Reference 4:
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-863

List Size:
400 ul

List Price:

$495.00

Datasheet
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