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Western blot analysis of extracts of various cell lines, using RPN2 antibody (23-526) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using RPN2 antibody (23-526) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using RPN2 antibody (23-526) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using RPN2 antibody (23-526) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

RPN2 Antibody

CATALOG NUMBER: 23-526

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 360-540 of human RPN2 (NP_002942.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
Positive Control 1:
HepG2
Positive Control 2:
293T
Positive Control 3:
Jurkat
Positive Control 4:
MCF7
Positive Control 5:
NIH/3T3
Positive Control 6:
Mouse liver
Predicted Molecular Weight:
Observed: 69kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RPN2
Additional Names:
RPN2, RIBIIR, RPN-II, RPNII, SWP1
Ncbi Gene Id Number:
6185
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-526

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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