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Western blot analysis in Hela cell line lysates (35ug/lane).
Western blot analysis in Hela cell line lysates (35ug/lane).

RPS14 Antibody

CATALOG NUMBER: 58-225

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Hamster, Drosophila, Mouse, Rat
Immunogen:
This RPS14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-147 amino acids from the C-terminal region of human RPS14.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
16 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RPS14
Additional Names:
40S ribosomal protein S14, RPS14
Protein Accession Number:
P62263
Protein Gi Number:
50403752
Ncbi Gene Id Number:
6208
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Ribosomes, the organelles that catalyze protein synthesis,consist of a small 40S subunit and a large 60S subunit. Togetherthese subunits are composed of 4 RNA species and approximately 80structurally distinct proteins. This gene encodes a ribosomalprotein that is a component of the 40S subunit. The protein belongsto the S11P family of ribosomal proteins. It is located in thecytoplasm. Transcript variants utilizing alternative transcriptioninitiation sites have been described in the literature. As istypical for genes encoding ribosomal proteins, there are multipleprocessed pseudogenes of this gene dispersed through the genome. InChinese hamster ovary cells, mutations in this gene can lead toresistance to emetine, a protein synthesis inhibitor. Multiplealternatively spliced transcript variants encoding the same proteinhave been found for this gene.
Background Reference 1:
Oliva, E.N., et al. Eur. J. Haematol. 85(3):231-235(2010)
Background Reference 2:
Borze, I., et al. Cancer Genet. Cytogenet. 197(2):166-173(2010)
Background Reference 3:
Quarello, P., et al. Haematologica 95(2):206-213(2010)
Background Reference 4:
Valencia, A., et al. Blood 112 (3), 918 (2008) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-225

List Size:
400 ul

List Price:

$495.00

Datasheet
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