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46-322 (0.3ug/ml) staining of lysate of cell line K562 (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-322 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-322 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-322 (5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-322 (0.3ug/ml) staining of lysate of cell line K562 (35ug protein in RIPA buffer). Detected by chemiluminescence.

RPS19 Antibody

CATALOG NUMBER: 46-322

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-KMVEKDQDGGRK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 16kDa band observed in lysate of cell line K562 (calculated MW of 16.1kDa according to NP_001013.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex), Liver and Tonsil. Recommended concentration: 5ug/ml.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RPS19
Additional Names:
RPS19, ribosomal protein S19, HGNC:10402, DBA, 40S ribosomal protein S19
Protein Accession Number:
NP_001013.1
Protein Gi Number:
4506695
Ncbi Gene Id Number:
6223
Background Reference 1:
Leger-Silvestre I, Caffrey JM, Dawaliby R, Alvarez-Arias DA, Gas N, Bertolone SJ, Gleizes PE, Ellis SR. Specific role for yeast homologs of the diamond blackfan anemia associated Rps19 protein in ribosome synthesis. J Biol Chem. 2005 Sep 12;

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-322

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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