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Western blot analysis in Hela,A2058 cell line lysates (15ug/lane).
RPS3A Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Hela,A2058 cell line lysates (15ug/lane).

RPS3A Antibody

CATALOG NUMBER: 61-964

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Bovine, Monkey, Mouse, Rat
Immunogen:
This RPS3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-254 amino acids from the C-terminal region of human RPS3A.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
30 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RPS3A
Additional Names:
40S ribosomal protein S3a {ECO:0000255, HAMAP-Rule:MF_03122}, v-fos transformation effector protein, Fte-1, RPS3A {ECO:0000255, HAMAP-Rule:MF_03122}, FTE1, MFTL
Protein Accession Number:
P61247
Protein Gi Number:
47117764
Ncbi Gene Id Number:
6189
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Ribosomes, the organelles that catalyze protein synthesis,consist of a small 40S subunit and a large 60S subunit. Togetherthese subunits are composed of 4 RNA species and approximately 80structurally distinct proteins. This gene encodes a ribosomalprotein that is a component of the 40S subunit. The protein belongsto the S3AE family of ribosomal proteins. It is located in thecytoplasm. Disruption of the gene encoding rat ribosomal proteinS3a, also named v-fos transformation effector protein, inv-fos-transformed rat cells results in reversion of the transformedphenotype. Transcript variants utilizing alternative transcriptionstart sites have been described. This gene is co-transcribed withthe U73A and U73B small nucleolar RNA genes, which are located inits fourth and third introns, respectively. As is typical for genesencoding ribosomal proteins, there are multiple processedpseudogenes of this gene dispersed through the genome.
Background Reference 1:
Maggi, L.B. Jr., et al. Mol. Cell. Biol. 28(23):7050-7065(2008)
Background Reference 2:
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Background Reference 3:
Jonson, L., et al. Mol. Cell Proteomics 6(5):798-811(2007)
Background Reference 4:
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-964

List Size:
400 ul

List Price:

$495.00

Datasheet
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