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Western blot analysis of lysate from HeLa cell line, using RPS6 Antibody(Ser240/244) at 1:1000 at each lane.
RPS6 antibody (Ser240/244) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPS6 antibody (Ser240/244) for immunohistochemistry.
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysate from HeLa cell line, using RPS6 Antibody(Ser240/244) at 1:1000 at each lane.

RPS6 Antibody

CATALOG NUMBER: 55-118

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Monkey, Mouse, Rat, Xenopus
Immunogen:
This RPS6 (Ser240/244) Antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-249 amino acids from human RPS6.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
29 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RPS6
Additional Names:
40S ribosomal protein S6, Phosphoprotein NP33, RPS6
Protein Accession Number:
P62753
Protein Gi Number:
51338632
Ncbi Gene Id Number:
6194
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Ribosomes, the organelles that catalyze protein synthesis,consist of a small 40S subunit and a large 60S subunit. Togetherthese subunits are composed of 4 RNA species and approximately 80structurally distinct proteins. This gene encodes a cytoplasmicribosomal protein that is a component of the 40S subunit. Theprotein belongs to the S6E family of ribosomal proteins. It is themajor substrate of protein kinases in the ribosome, with subsets offive C-terminal serine residues phosphorylated by different proteinkinases. Phosphorylation is induced by a wide range of stimuli,including growth factors, tumor-promoting agents, and mitogens.Dephosphorylation occurs at growth arrest. The protein maycontribute to the control of cell growth and proliferation throughthe selective translation of particular classes of mRNA. As istypical for genes encoding ribosomal proteins, there are multipleprocessed pseudogenes of this gene dispersed through the genome.
Background Reference 1:
Maggi, L.B. Jr., et al. Mol. Cell. Biol. 28(23):7050-7065(2008)
Background Reference 2:
Fujita, K., et al. Acta Neuropathol. 116(4):439-445(2008)
Background Reference 3:
Robledo, S., et al. RNA 14(9):1918-1929(2008)
Background Reference 4:
Glover, E.I., et al. Am. J. Physiol. Regul. Integr. Comp. Physiol. 295 (2), R604-R610 (2008) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-118

List Size:
400 ul

List Price:

$495.00

Datasheet
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