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Western blot analysis of extracts of various cell lines, using RPS6KA2 antibody (16-539) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using RPS6KA2 antibody (16-539) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

RPS6KA2 Antibody

CATALOG NUMBER: 16-539

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 632-733 of human RPS6KA2 (NP_066958.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:100 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
A-549
Positive Control 3:
U-87MG
Positive Control 4:
PC-12
Positive Control 5:
Mouse brain
Positive Control 6:
Mouse heart
Predicted Molecular Weight:
Observed: 83kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
RPS6KA2
Additional Names:
ribosomal protein S6 kinase, 90kD, polypeptide 2, ribosomal protein S6 kinase alpha 2, ribosomal S6 kinase 3, pp90RSK3, p90-RSK3, S6K-alpha2, S6K-alpha, RSK3, RSK, MAPKAPK1C, HU-2, ribosomal protein S6 kinase, 90kDa, RPS6KA2
Ncbi Gene Id Number:
6196
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains two non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternative splice variants, encoding different isoforms, have been characterized.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-539

List Size:
100 uL

List Price:

$436.00

Datasheet
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