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Western blot analysis in Ramos cell line and mouse thymus tissue lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with RSK3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis in Ramos cell line and mouse thymus tissue lysates (35ug/lane).

RSK2 Antibody

CATALOG NUMBER: 63-300

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This RSK2 (RPS6KA3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human RSK2 (RPS6KA3).
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
84 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RPS6KA3
Additional Names:
Ribosomal protein S6 kinase alpha-3, S6K-alpha-3, 90 kDa ribosomal protein S6 kinase 3, p90-RSK 3, p90RSK3, Insulin-stimulated protein kinase 1, ISPK-1, MAP kinase-activated protein kinase 1b, MAPK-activated protein kinase 1b, MAPKAP kinase 1b, MAPKAPK-1b, Ribosomal S6 kinase 2, RSK-2, pp90RSK2, RPS6KA3, ISPK1, MAPKAPK1B, RSK2
Protein Accession Number:
P51812
Protein Gi Number:
1730070
Ncbi Gene Id Number:
6197
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RSK3 is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in the gene have been associated with Coffin-Lowry syndrome (CLS).
Background Reference 1:
Yang, X., et al., Cell 117(3):387-398 (2004).
Background Reference 2:
Guimiot, F., et al., Gene Expr. Patterns 4(1):111-114 (2004).
Background Reference 3:
Zeniou, M., et al., (er) Nucleic Acids Res. 32(3):1214-1223 (2004).
Background Reference 4:
Vaidyanathan, H., et al., J. Biol. Chem. 278(34):32367-32372 (2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-300

List Size:
400 ul

List Price:

$495.00

Datasheet
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