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Western blot analysis of lysate from 293 cell line, using RSPO2 Antibody at 1:1000 at each lane.
RSPO2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysate from 293 cell line, using RSPO2 Antibody at 1:1000 at each lane.

RSPO2 Antibody

CATALOG NUMBER: 61-862

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This RSPO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 190-218 amino acids from the C-terminal region of human RSPO2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
28 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RSPO2
Additional Names:
R-spondin-2, Roof plate-specific spondin-2, hRspo2, RSPO2
Protein Accession Number:
Q6UXX9
Protein Gi Number:
147744588
Ncbi Gene Id Number:
340419
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
R-spondins (RSPOs), such as RSPO2, are secreted proteins that regulate beta-catenin (CTNNB1; MIM 116806) signaling.
Background Reference 1:
Kim, K.A., et al. Cell Cycle 5(1):23-26(2006)
Background Reference 2:
Kazanskaya, O., et al. Dev. Cell 7(4):525-534(2004)
Background Reference 3:
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-862

List Size:
400 ul

List Price:

$495.00

Datasheet
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