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Western blot analysis of RSPO3 in 293 cell lysate with RSPO3 antibody at 1 μg/ml.
Immunocytochemistry of RSPO3 in 293 cells with RSPO3 antibody at 5 μg/mL.
Immunofluorescence of RSPO3 in 293 cells with RSPO3 antibody at 20 μg/mL.
Western blot analysis of RSPO3 in 293 cell lysate with RSPO3 antibody at 1 μg/ml.
Trial Size

RSPO3 Antibody

CATALOG NUMBER: 8153

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
RSPO3 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human RSPO3.
The immunogen is located within amino acids 200 - 250 of RSPO3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
RSPO3 antibody can be used for detection of RSPO3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
RSPO3 antibody is human and mouse reactive. At least three isoforms of RSPO3 are known to exist; this antibody will detect all three.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 17-010 - HEK293 Cell Slide
Predicted Molecular Weight:
Predicted: 30 kDa
Observed: 30 kDa
Purification:
RSPO3 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RSPO3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RSPO3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
RSPO3
Additional Names:
R-spondin-3, CRISTIN1, PWTSR, THSD2
Protein Accession Number:
NP_116173
Protein Gi Number:
18490982
Ncbi Gene Id Number:
84870
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RSPO3 is a member of the R-spondin family and plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis (1,2). RSPO3 has been shown to be involved in regulating the balance between angioblast and blood cell specification during embryonic vasculogenesis and angiogenesis and may be involved in tumor development (3,4).
Background Reference 1:
Chen JZ, Wang S, Tang R, et al. Cloning and identification of a cDNA that encodes a novel human protein with thrombospondin type I repeat domain, hPWTSR. Mol. Biol. Rep. 2002; 29:287-92.
Background Reference 2:
Kim KA, Wagle M, Tran K, et al. R-Spondin family members regulate the Wnt pathway by a common mechanism. Mol. Biol. Cell 2008; 19:2588-96.
Background Reference 3:
Kazanskaya O, Ohkawara B, Heroult M, et al. The Wnt signaling regulator R-spondin 3 promotes angioblast and vascular development. Development 2008; 3655-64.
Background Reference 4:
Seshagiri S, Stawiski EW, Durinck S, et al. Recurrent R-spondin fusions in colon cancer. Nature 2012; 488:660-4.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8153

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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