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Human R-Spondin 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Human R-Spondin 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Rspondin 1 Recombinant Protein

CATALOG NUMBER: 96-658

Tested Applications:
WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Ser 21 - Ala 263
Fusion Tag:
His Tag
Tested Applications:
WB
Application Note:
This recombinant protein can be used for WB. For research use only.
Predicted Molecular Weight:
28.7 kDa
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20μg/ml (100μl/well) can bind human LIMPII with a linear ranger of 31. 25 - 1000 ng/mL.
Purity:
>95% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
PBS, pH7.4
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
RSPO1
Additional Names:
RSPO1, CRISTIN3, FLJ40906, RP11-566C13.1, R-spondin-1
Protein Accession Number:
NP_001033722.1
Ncbi Gene Id Number:
284654
Background:
R-spondin-1 is also known as Roof plate-specific Spondin 1 (RSPO1) and cysteinerich and single thrombospondin domain containing protein 3 (Cristin 3), which is a secreted protein which belongs to the R-Spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. All Rspondins regulate Wnt/β-catenin signaling, but have distinct expression patterns. Like other R-Spondins, R-Spondin-1 contains two adjacent cysteinerich furinlike domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furinlike domains are needed for β-catenin stabilization. A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus.
Background Reference 1:
Chen, JZ. et al. ,2002, Mol Biol Rep. 2002;29:287.
Background Reference 2:
Kim, K.A. et al., 2006, Cell Cycle. 2006;5:23–26.
Background Reference 3:
Nam, J.S. et al., 2007, Gene Expr. Patterns 7, 306–312.
Background Reference 4:
Kazanskaya, O. et al., 2004, Dev. Cell 7:525.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

96-658

List Size:
0.05 mg

List Price:

$626.00

Datasheet
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