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Western blot analysis of RTP801 in human kidney tissue lysate with RTP801 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of RTP801 in mouse kidney tissue with RTP801 antibody at 5 μg/mL.
Immunofluorescence of RTP801 in mouse kidney tissue with RTP801 antibody at 20 μg/mL.<br><br>Red: RTP801 Antibody (4495) <br> Blue: DAPI staining
Western blot analysis of RTP801 in human kidney tissue lysate with RTP801 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

RTP801 Antibody

CATALOG NUMBER: 4495

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
RTP801 antibody was raised against a 12 amino acid synthetic peptide from near the center of human RTP801.
The immunogen is located within amino acids 90 - 140 of RTP801.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
RTP801 antibody can be used for the detection of RTP801 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
RTP801 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RTP801 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RTP801 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DDIT4
Additional Names:
RTP801 Antibody: Dig2, REDD1, REDD-1, RTP801, DNA damage-inducible transcript 4 protein, HIF-1 responsive protein RTP801
Protein Accession Number:
NP_061931
Protein Gi Number:
9506687
Ncbi Gene Id Number:
54541
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RTP801 Antibody: RTP801 was initially identified as a gene induced by DNA damage, and later found to also be regulated by other cellular stresses such as hypoxia and glucocorticoid treatment. Recently, RTP801 has been shown to act as a mediator of tuberous sclerosis complex (TSC)-dependent regulation of the mammalian Target of Rapamycin (mTOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle. In response to energy stress, RTP801 inhibits mTOR function, resulting in dephosphorylation of downstream targets such as ribosomal protein S6 kinase 1 and 4EBP1 and decreasing cell growth. Disregulation of RTP801 may thus contribute to human tumorigenesis.
Background Reference 1:
Ellisen LW, Ramsayer KD, Johannessen CM, et al. REDD1, a developmentally regulated transcriptional target of p63 and p53, links p63 to regulation of reactive oxygen species. Mol. Cell2002; 10:995-1005.
Background Reference 2:
Shoshani T, Faerman A, Mett I, et al. Identification of a novel hypoxia-inducible factor 1-responsive gene, RTP801, involved in apoptosis. Mol. Cell. Biol.2002; 22:2283-93.
Background Reference 3:
Wang Z, Malone MH, Thomenius MJ, et al. Dexamethasone-induced gene 2 (dig2) is a novel pro-survival stress gene induced rapidly by diverse apoptotic signals. J. Biol. Chem.2003; 278:27053-8.
Background Reference 4:
Sofer A, Lei K, Johannessen CM, et al. Regulation of mTOR and cell growth in response to energy stress by REDD1. Mol. Cell. Biol.2005; 25:5834-45.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4495

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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