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Western blot analysis of Rubicon in 293 cell lysate with Rubicon antibody at 1 μg/ml.
Immunohistochemistry of RUBICON in human kidney tissue with RUBICON antibody at 5 μg/ml.
Immunofluorescence of RUBICON in human kidney tissue with RUBICON antibody at 20 μg/ml.
Western blot analysis of Rubicon in 293 cell lysate with Rubicon antibody at 1 μg/ml.
Trial Size

Rubicon Antibody

CATALOG NUMBER: 7943

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Rubicon antibody was raised against a 16 amino acid peptide near the amino terminus of human Rubicon.
The immunogen is located within amino acids 240 - 290 of Rubicon.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
Rubicon body can be used for detection of Rubicon by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
Rubicon antibody is human, mouse and rat reactive. Multiple isoforms of Rubicon are known to exist.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Predicted Molecular Weight:
Predicted: 90, 101, 109 kDa
Observed: 91, 99 kDa
Purification:
Rubicon antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Rubicon antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Rubicon antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
KIAA0226
Additional Names:
Run domain Beclin-1 interacting and cysteine-rich containing protein, KIAA0226
Protein Accession Number:
XP_005269431
Protein Gi Number:
530375960
Ncbi Gene Id Number:
9711
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation (1). Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex (2). Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines (2).
Background Reference 1:
Matsunaga K, Saitoh T, Tabata K, et al. Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages. Nat. Cell Biol. 2009; 11:385-96.
Background Reference 2:
Yang CS, Lee JS, Rodgers M, et al. Autophagy protein Rubicon mediates phagocytic NAPDH oxidase activation in response to microbial infection of TLR stimulation. Cell Host and Microbe 2012; 11:264-76.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7943

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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