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Western blot analysis of RUNX1 in Raji cell lysate with RUNX1 antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of RUNX1 in Raji cell lysate with RUNX1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

RUNX1 Antibody

CATALOG NUMBER: 5149

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
RUNX1 antibody was raised against a 16 amino acid synthetic peptide from near the center of human RUNX1.
The immunogen is located within amino acids 160 - 210 of RUNX1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
RUNX1 antibody can be used for detection of RUNX1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1207 - Raji Cell Lysate
Purification:
RUNX1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RUNX1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RUNX1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RUNX1
Additional Names:
RUNX1 Antibody: AML1, CBFA2, EVI-1, AMLCR1, PEBP2aB, AML1-EVI-1, AML1, Runt-related transcription factor 1, Acute myeloid leukemia 1 protein, CBF-alpha-2
Protein Accession Number:
AAI36381
Protein Gi Number:
223459612
Ncbi Gene Id Number:
861
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RUNX1 Antibody: RUNX1 is one of three mammalian RUNX genes that control multiple aspects of embryonic development and are responsible for the pathogenesis of many human diseases. RUNX1 plays major roles in the development of nociceptive sensory neurons in addition to hematopoietic stem cells (HSC) with the exception of the erythroid lineage. During development, Notch signals mediate RUNX1 induction with SCL/GATA/Ets factors, and Wnt signals potentially cooperate with RUNX1 to facilitate adult HSC expansion via cooperative induction of cyclin D, cdk4, and other cell cycle regulators. In turn, RUNX1 regulates cell cycle transitions dependent on functional/physical interactions with other proteins such as HDAC1 and -3, mSin3A, p300, SMAD proteins, and LEF/TCF.
Background Reference 1:
Lund AH and van Lohuizen M. RUNX: a trilogy of cancer genes. Cancer Cell2002; 1:213-5.
Background Reference 2:
Inoue K, Shiga T, and Ito Y. Runx transcription factors in neuronal development. Neur. Dev.2008; 3:20.
Background Reference 3:
North TE, Stacy T, Matheny CJ, et al. Runx1 is expressed in adult mouse hematopoietic stem cells and differentiating myeloid and lymphoid cells, but not in maturing erythroid cells. Stem Cells2004; 22:158-68.
Background Reference 4:
Lee TI, Jenner RG, Boyer LA. Control of developmental regulators by Polycomb in human embryonic stem cells. Cell2006; 125:301-13.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5149

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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