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Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Antibody used in WB on Human 721_B at 1 ug/ml.
Antibody used in WB on Human HepG2 at 1.25 ug/ml.

RUNX1T1 Antibody

CATALOG NUMBER: 27-986

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat, Zebrafish
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat, Zebrafish
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RUNX1T1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
RUNX1T1 antibody can be used for detection of RUNX1T1 by ELISA at 1:312500. RUNX1T1 antibody can be used for detection of RUNX1T1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
62 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store RUNX1T1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
RUNX1T1
Additional Names:
RUNX1T1, CDR, ETO, MTG8, AML1T1, ZMYND2, CBFA2T1
Protein Accession Number:
NP_783554
Protein Gi Number:
28329422
Ncbi Gene Id Number:
862
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RUNX1T1 is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t (8;21) (q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation.
Background Reference 1:
Lausen, J., et al., (2004) J. Biol. Chem. 279 (47), 49281-49288.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-986

List Size:
100 ul

List Price:

$473.00

Datasheet
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