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Western blot analysis of lysates from HL-60, Jurkat cell line (from left to right), using RUNX3 Antibody at 1:1000 at each lane.
RUNX3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Fluorescent confocal image of Hela cell stained with RUNX3 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RUNX3 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RUNX3 immunoreactivity is localized to Nucleus and Cytoplasm significantly.
Western blot analysis of lysates from HL-60, Jurkat cell line (from left to right), using RUNX3 Antibody at 1:1000 at each lane.

RUNX3 Antibody

CATALOG NUMBER: 57-901

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This RUNX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-197 amino acids from the Central region of human RUNX3.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
44 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RUNX3
Additional Names:
Runt-related transcription factor 3, Acute myeloid leukemia 2 protein, Core-binding factor subunit alpha-3, CBF-alpha-3, Oncogene AML-2, Polyomavirus enhancer-binding protein 2 alpha C subunit, PEA2-alpha C, PEBP2-alpha C, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit, RUNX3, AML2, CBFA3, PEBP2A3
Protein Accession Number:
Q13761
Protein Gi Number:
17368453
Ncbi Gene Id Number:
864
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the runt domain-containingfamily of transcription factors. A heterodimer of this protein anda beta subunit forms a complex that binds to the core DNA sequence5'-PYGPYGGT-3' found in a number of enhancers and promoters, andcan either activate or suppress transcription. It also interactswith other transcription factors. It functions as a tumorsuppressor, and the gene is frequently deleted or transcriptionallysilenced in cancer. Multiple transcript variants encoding differentisoforms have been found for this gene.
Background Reference 1:
Tsang, Y.H., et al. Oncogene 29(41):5643-5650(2010)
Background Reference 2:
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Background Reference 3:
Iwatani, K., et al. Biochem. Biophys. Res. Commun. 400(3):426-431(2010)
Background Reference 4:
Kodach, L.L., et al. Carcinogenesis 31(9):1567-1575(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-901

List Size:
400 ul

List Price:

$519.00

Datasheet
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