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Western blot analysis of RUSC1 in A-20 cell lysate with RUSC1 antibody at 1 μg/mL.
Western blot analysis of RUSC1 in A-20 cell lysate with RUSC1 antibody at 1 μg/mL.
Trial Size

RUSC1 Antibody

CATALOG NUMBER: 5411

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
RUSC1 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human RUSC1.
The immunogen is located within amino acids 780 - 830 of RUSC1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
RUSC1 antibody can be used for detection of RUSC1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Purification:
RUSC1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RUSC1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RUSC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RUSC1
Additional Names:
RUSC1 Antibody: NESCA, NESCA, RUN and SH3 domain-containing protein 1, New molecule containing SH3 at the carboxy-terminus, Nesca
Protein Accession Number:
NP_001098673
Protein Gi Number:
157412245
Ncbi Gene Id Number:
23623
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RUSC1 Antibody: RUSC1, also known as NESCA, shares with the related protein RUSC2 a common domain structure of RUN, leucine zipper and SH3 domain in addition to over 30% amino acid identity. RUSC1 is an adapter protein that can bind to the TrkA receptor and is necessary in the NGF-induced neurite growth of PC12 cells. RUSC1 has also been shown to interact with IκB kinase- (IKK-) gamma, the regulatory subunit of the IKK complex that is required for NF-κB activation in many signaling pathways such as TNF-R or the TLR pathways. RUSC1 can also bind to the E3 ubiquitin ligase TRAF6, which then catalyzes RUSC1 polyubiquitination. Since overexpression of RUSC1 strongly inhibits TRAF6-mediated polyubiquitination of IKK-gamma, RUSC1 may be a link in the IKK-gamma-mediated NF-κB activation pathway.
Background Reference 1:
Matsuda S, Miyazaki K, Ichigotani Y, et al. Molecular cloning and characterization of a novel human gene (NESCA) which encodes a putative adapter protein containing SH3. Biochim. Biophys. Acta2000; 1491:321-6.
Background Reference 2:
Katoh M and Katoh M. Characterization of RUSC1 and RUSC2 genes in silico. Oncol. Rep.2004; 12:933-8.
Background Reference 3:
MacDonald JI, Kubu CJ, and Meakin SO. Nesca, a novel adapter, translocates to the nuclear envelope and regulates neurotrophin-induced neurite outgrowth. J. Cell Biol.2004; 164:851-62.
Background Reference 4:
Napolitano G, Mirra S, Monfregola J, et al. NESCA: a new NEMO/IKKgamma and TRAF6 interacting protein. J. Cell. Phys.2009; 220:410-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5411

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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