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Western blot analysis of RUSC2 in SK-N-SH cell lysate with RUSC2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Immunohistochemistry of RUSC2 in human brain tissue with RUSC2 antibody at 5 μg/mL.
Immunofluorescence of RUSC2 in human brain tissue with RUSC2 antibody at 20 μg/mL.
Western blot analysis of RUSC2 in SK-N-SH cell lysate with RUSC2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Trial Size

RUSC2 Antibody

CATALOG NUMBER: 6659

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
RUSC2 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human RUSC2.
The immunogen is located within amino acids 70 - 120 of RUSC2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
RUSC2 antibody can be used for detection of RUSC2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms are known to exist; this antibody will detect all three isoforms. This antibody is predicted to not cross-react with RUSC1.
Positive Control 1:
Cat. No. 1220 - SK-N-SH Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
RUSC2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RUSC2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RUSC2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RUSC2
Additional Names:
RUSC2 Antibody: Iporin, KIAA0375, Iporin, Interacting protein of Rab1
Protein Accession Number:
NP_055621
Protein Gi Number:
55741719
Ncbi Gene Id Number:
9853
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RUSC2 Antibody: RUSC2, also known as Iporin, shares with the related protein RUSC1 a common domain structure of RUN, leucine zipper and SH3 domain in addition to over 30% amino acid identity. RUSC2 is a rab1-interacting protein that also interacts with GM130, another rab1-interacting protein. RUSC2 interacts with specific rab1 isoforms with different rab-binding specificity. It has been suggested that RUSC2 may function as a link between the targeting of ER derived vesicles triggered by the rab1 GTPase and a signaling pathway composed of proteins containing SH3 and/or poly-proline regions.
Background Reference 1:
Bayer M, Fischer J, Kremerskthen J, et al. Identification and characterization of Iporin as a novel interaction partner for rab1. BMC Cell Biol. 2005; 29:6:15.
Background Reference 2:
Katoh M and Katoh M. Characterization of RUSC1 and RUSC2 genes in silico. Oncol. Rep. 2004; 12:933-8
Background Reference 3:
Fukuda M, Kobayashi H, Ishibashi K, et al. Genome-wide investigation of the rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35. Cell Struct. Funct. 2011; 36:155-70

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6659

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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