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43-130 (2ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-130 (2ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

RUTBC3 Antibody

CATALOG NUMBER: 43-130

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: RDHENYVACSRSHRR
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx. 85kDa band observed in lysates of Human Lung carcinoma cell line A549 (calculated MW of 85.4kDa according to NP_056520.2). Recommended concentration: 2-3ug/ml.
Predicted Molecular Weight:
Approx 85kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RUTBC3
Additional Names:
SGSM3, small G protein signaling modulator 3, DKFZp761D051, MAP, RUSC3, RUTBC3, RUN and SH3 containing 3, RUN and TBC1 domain containing 3, merlin binding protein, merlin-associated protein, small G protein signaling modulator 3 protein
Protein Accession Number:
NP_056520.2
Protein Gi Number:
168985143
Ncbi Gene Id Number:
27352
Background Reference 1:
Lee IK, Kim KS, Kim H, Lee JY, Ryu CH, Chun HJ, Lee KU, Lim Y, Kim YH, Huh PW, Lee KH, Han SI, Jun TY, Rha HK.MAP, a protein interacting with a tumor suppressor, merlin, through the run domain.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-130

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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