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Western blot analysis of RUVBL1 Antibody in MCF-7 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with RUVBL1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of RUVBL1 using rabbit polyclonal RUVBL1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RUVBL1 gene.
Western blot analysis of RUVBL1 Antibody in MCF-7 cell line lysates (35ug/lane)

RUVBL1 Antibody

CATALOG NUMBER: 60-402

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This RUVBL1 antibody is generated from rabbits immunized with human RUVBL1 recombinant protein.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RUVBL1
Additional Names:
RuvB-like 1, 49 kDa TATA box-binding protein-interacting protein, 49 kDa TBP-interacting protein, 54 kDa erythrocyte cytosolic protein, ECP-54, INO80 complex subunit H, Nuclear matrix protein 238, NMP 238, Pontin 52, TIP49a, TIP60-associated protein 54-alpha, TAP54-alpha, RUVBL1, INO80H, NMP238, TIP49, TIP49A
Protein Accession Number:
Q9Y265
Protein Gi Number:
28201891
Ncbi Gene Id Number:
8607
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RUVBL1 possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. It is a component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1 -CTNNB1 complex. High levels of autoantibodies against RUVBL1 are detected in sera of patients with autoimmune diseases such as polymyositis/dermatomyosistis and autoimmune hepatitis.
Background Reference 1:
Feng, Y., et al., Cancer Res. 63(24):8726-8734 (2003).
Background Reference 2:
Hawley, S.B., et al., J. Biol. Chem. 276(1):179-186 (2001).
Background Reference 3:
Ikura, T., et al., Cell 102(4):463-473 (2000).
Background Reference 4:
Salzer, U., et al., Biochim. Biophys. Acta 1446(3):365-370 (1999).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-402

List Size:
400 ul

List Price:

$495.00

Datasheet
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