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Western blot analysis of RWDD3 in rat kidney tissue lysate with RWDD3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of RWDD3 in rat kidney tissue lysate with RWDD3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

RWDD3 Antibody

CATALOG NUMBER: 5407

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (93%)
Immunogen:
RWDD3 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human RWDD3.
The immunogen is located within the last 50 amino acids of RWDD3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
RWDD3 antibody can be used for detection of RWDD3 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1465 - Rat Kidney Tissue Lysate
Purification:
RWDD3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RWDD3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RWDD3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RWDD3
Additional Names:
RWDD3 Antibody: RSUME, RSUME, RWD domain-containing protein 3, RWD-containing sumoylation enhancer
Protein Accession Number:
NP_056300
Protein Gi Number:
153252154
Ncbi Gene Id Number:
25950
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RWDD3 Antibody: RWDD3 (RSUME), a small RWD-containing protein, has a central role in sumoylation by enhancing SUMO conjugation in the regulatory network of immune-inflammatory signals. RWDD3 increases IκBeta sumoylation and stability. In addition, RWDD3 inhibits TNF-α-induced kappaB-LUC (Luciferase) reporter activity, showing the functional consequence of IκBeta increased stability. RSUME-enhanced sumoylation of IκBeta leads to the inhibition of NF-κBeta activity on two well-known inflammatory genes, IL-8 and cyclooxygenase-2 (Cox-2) and therefore may also favor anti-inflammatory pathways. Expression of RWDD3 was induced under hypoxic conditions and it has a potential role during vascularization. Both BMP-4 and RWDD3 may be interesting targets for inhibiting steps involved in pituitary tumorigenesis.
Background Reference 1:
Carbia-Nagashima A, Gerez J, Perez-Castro C, et al. RSUME, a small RWD-containing protein, enhances SUMO conjugation and stabilizes HIF-1alpha during hypoxia. Cell2007; 131:309-23.
Background Reference 2:
Liberman AC, Druker J, Garcia FA, et al. Intracellular molecular signaling. Basis for specificity to glucocorticoid anti-inflammatory actions. Ann. NY Acad. Sci.2009; 1153:6-13.
Background Reference 3:
Giacomini D, Haedo M, Gerez J, et al. Differential gene expression in models of pituitary prolactin-producing tumoral cells. Horm. Res.2009; 71 Suppl 2:88-94.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5407

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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