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45-139 (0.3ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-139 (0.3ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

RXR alpha Antibody

CATALOG NUMBER: 45-139

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: CQVNSSLTSPTGRGSM
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 50.8kDa according to NP_002948.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Solomon et al, J Biol Chem. 1998 Jul 10;273(28):17573-8; PMID: 9651351. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on NIH3T3 cell line for this particular batch.
Specificity:
This antibody is expected to recognise an epitope corresponding to aa 14-28 of human RXR alpha protein and does not cross-react with either RXR beta or gamma.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
RXRA
Additional Names:
RXRA, NR2B1, retinoid X receptor, alpha, FLJ16020, FLJ16733, MGC102720, FLJ00280, FLJ00318,
Protein Accession Number:
NP_002948.1
Protein Gi Number:
4506755
Ncbi Gene Id Number:
6256
Background Reference 1:
Claudel T, Leibowitz MD, Fievet C, Tailleux A, Wagner B, Repa JJ, Torpier G, Lobaccaro JM, Paterniti JR, Mangelsdorf DJ, Heyman RA, Auwerx J. Reduction of atherosclerosis in apolipoprotein E knockout mice by activation of the retinoid X receptor. Proc Natl Acad Sci USA. 2001 Feb 27;98(5):2610-5. Epub 2001 Feb 20.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-139

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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