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Western blot analysis of lysate from mouse NIH/3T3 cell line, using RYK Antibody (N175) at 1:1000.
Western blot analysis of lysate from human ovary tissue lysate, using RYK Antibody (N175) at 1:1000.
Western blot analysis of lysate from human ovary tissue lysate, using RYK Antibody (N175) at 1:1000.
Western blot analysis of lysates from mouse lung and ovary tissue lysate(from left to right), using RYK Antibody (N175) at 1:1000 at each lane.
Antibody is used in Western blot to detect RYK in Jurkat cell lysate.
Western blot analysis of RYK using rabbit polyclonal RYK Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RYK gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of lysate from mouse NIH/3T3 cell line, using RYK Antibody (N175) at 1:1000.

RYK Antibody

CATALOG NUMBER: 63-134

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
This RYK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-190 amino acids from human RYK.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
68 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RYK
Additional Names:
Tyrosine-protein kinase RYK, RYK, JTK5A
Protein Accession Number:
P34925
Protein Gi Number:
1710811
Ncbi Gene Id Number:
6259
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RYK is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. A nine nucleotide insertion in some transcripts results in the SLG variant. It is not established whether this is a product of alternative splicing or a second gene, since evidence for a second gene or pseudogene on chromosome 17 exists.
Background Reference 1:
Trivier, E., et al., J. Biol. Chem. 277(25):23037-23043 (2002). Katso, R.M., et al., Mol. Cell. Biol. 19(9):6427-6440 (1999). Wang, X.C., et al., Mol. Med. 2(2):189-203 (1996). Tamagnone, L., et al., Oncogene 8(7):2009-2014 (1993). Stacker, S.A., et al., Oncogene 8(5):1347-1356 (1993).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-134

List Size:
400 ul

List Price:

$495.00

Datasheet
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