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Western blot analysis using S100 A antibody against truncated S100A recombinant protein.
Immunohistochemical analysis of paraffin - embedded human brain tissue showing cytoplasmic using S100A antibody with DAB staining.
Western blot analysis using S100 A antibody against truncated S100A recombinant protein.

S100A Antibody [2C8B8 / 2C8E8]

CATALOG NUMBER: 32-213

Clonality:
Monoclonal
Tested Applications:
ELISA
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant S100A expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Dilution
ELISA:Propose dilution 1:10,000
Determining optimal working dilutions by titration test.
Purification:
Antibody is purified by protein A affinity chromatography.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
S100A monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
S100A1
Additional Names:
S100A, S-100 protein alpha chainS100, S100A, S100-alpha
Protein Accession Number:
P23297
Protein Gi Number:
134136
Ncbi Gene Id Number:
6271
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
S100 calcium binding protein A1 (S100-alpha/ S100A1), The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies.
Background Reference 1:
Koenig A, Wojcieszyn J, Weeks BR, et al. Vet Pathol. 2001;38(4):427-35.
Background Reference 2:
Hoyaux D, Decaestecker C, Heizmann CW, et al. Brain Res. 2000;867(1-2):280-8.
Background Reference 3:
Pingerelli PL, Mizukami H, Wagner AS, et al. J Protein Chem. 1990;9(2):169-75.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-213

List Size:
0.1 mL

List Price:

$432.00

Datasheet
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