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46-326 (0.05ug/ml) staining of Peripheral blood mononuclear cells lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-326 (2ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
46-326 (0.05ug/ml) staining of Peripheral blood mononuclear cells lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

S100A4 Antibody

CATALOG NUMBER: 46-326

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: CNEFFEGFPDKQP
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 13kDa band observed in Human Peripheral blood mononuclear cells lysates (calculated MW of 11.7kDa according to NP_002952.1). Recommended concentration: 0.05-0.2ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Small Intestine shows staining of lymphatic cells in the lamina propria. Recommended concentration: 1-2ug/ml.
Specificity:
Both variants (NP_002952.1; NP_062427.1) represent identical protein.
Positive Control 1:
Cat. No. 1309 - Human Placenta Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
S100A4
Additional Names:
S100A4, CAPL, S100 calcium binding protein A4, 18A2, 42A, MTS1, P9KA, PEL98, S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog), S100 calcium-binding protein A4, S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog), malignant transformation suppression 1
Protein Accession Number:
NP_002952.1,NP_062427.1
Protein Gi Number:
4506765
Ncbi Gene Id Number:
6275
Background Reference 1:
Li ZH, Bresnick AR. The S100A4 metastasis factor regulates cellular motility via a direct interaction with myosin-IIA. Cancer Res. 2006 May 15;66(10):5173-80.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-326

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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