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Western blot analysis of S100A6 antibody in mouse lung tissue lysates (35ug/lane)
Western blot analysis of S100A6 using rabbit polyclonal S100A6 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the S100A6 gene (Lane 2).
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of S100A6 antibody in mouse lung tissue lysates (35ug/lane)

S100A6 Antibody

CATALOG NUMBER: 62-845

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This S100A6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-67 amino acids from the C-terminal region of human S100A6.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
10 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
S100A6
Additional Names:
Protein S100-A6, Calcyclin, Growth factor-inducible protein 2A9, MLN 4, Prolactin receptor-associated protein, PRA, S100 calcium-binding protein A6, S100A6, CACY
Protein Accession Number:
P06703
Protein Gi Number:
116509
Ncbi Gene Id Number:
6277
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
S100A6 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis.
Background Reference 1:
van Dieck,J. J. Biol. Chem. 284 (20), 13804-13811 (2009)
Background Reference 2:
Tsoporis,J.N., Izhar,S. J. Biol. Chem. 283 (44), 30174-30183 (2008)
Background Reference 3:
Lee,Y.T., Dimitrova,Y.N. Biochemistry 47 (41), 10921-10932 (2008)
Background Reference 4:
Melle,C., Ernst,G. PLoS ONE(12), E3767 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-845

List Size:
400 ul

List Price:

$495.00

Datasheet
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