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Western blot analysis using anti - S100B monoclonal antiobdy against full - length S100B recombinant protein.
<b>Below:</b>Immunohistochemical analysis of paraffin - embedded human brain tissue (A) and human thymus tissue (B), showing cytoplasmic localization using anti - S100B antibody with DAB staining.
Western blot analysis using anti - S100B monoclonal antiobdy against full - length S100B recombinant protein.

S100B Antibody [9A11B9]

CATALOG NUMBER: 32-214

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Purified full-length recombinant S100B-GST expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:2,000 b<>IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
S100B monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
S100B
Additional Names:
S-100 protein beta chainNEF, S100, S100-B, S100beta
Protein Accession Number:
P04271
Protein Gi Number:
134138
Ncbi Gene Id Number:
6285
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
S100B (S100 calcium binding protein B) is a member of the S100 family of proteins containing 2 EF-hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S-100 proteins and parvalbumin proteins are each expressed in neural tissues. In addition, S100B are present in a variety of other tissues, and calbindin is present in intestine and kidney. Parvalbumin B is found in many tumor tissues as well as in the organ of Corti. Calbindin, S-100 proteins and parvalbulmins have all been detected in leydig cells and the testis. These proteins are thought to play a role in hormone production and spermatogenesis. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
Background Reference 1:
Shapiro LA. Marks A. Whitaker-Azmitia PM. Brain Res. 2004, Jun 4,1010(1-2):17-21.
Background Reference 2:
Sorci G. Riuzzi F. Arcuri C. et al. Mol Cell Biol. 2004, Jun,24(11):4880-94.
Background Reference 3:
Zimmer DB. Chaplin J. Baldwin A. et al. Cell Mol Biol (Noisy-le-grand).2005,Sep 5,51(2):201-14.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-214

List Size:
0.1 mL

List Price:

$495.00

Datasheet
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