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Western blot analysis of S1P1 in mouse thymus lysate with S1P1 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of S1P1 in mouse thymus tissue with S1P1 antibody at 5 μg/mL.
Immunohistochemistry of S1P1 in mouse thymus tissue with S1P1 antibody at 2.5 μg/ml.
Immunofluorescence of S1P1 in mouse thymus tissue with S1P1 antibody at 20 μg/mL.<br><br>Green: S1P1 Antibody (4809) <br> Blue: DAPI staining
Western blot analysis of S1P1 in mouse thymus lysate with S1P1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

S1P1 Antibody

CATALOG NUMBER: 4809

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (86%)
Immunogen:
S1P1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of the human S1P1.
The immunogen is located within the last 50 amino acids of S1P1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
S1P1 antibody can be used for detection of S1P1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of S1P1 are known to exist; this S1P1 antibody will only recognize the shorter isoform.
Positive Control 1:
Cat. No. 1409 - Mouse Thymus Tissue Lysate
Predicted Molecular Weight:
Predicted: 42 kDa
Observed: 42 kDa
Purification:
S1P1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
S1P1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
S1P1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
S1PR1
Additional Names:
S1P1 Antibody: EDG1, S1P1, CD363, ECGF1, EDG-1, CHEDG1, D1S3362, EDG1, Sphingosine 1-phosphate receptor 1, Endothelial differentiation G-protein coupled receptor 1, S1P receptor 1
Protein Accession Number:
NP_001391
Protein Gi Number:
13027636
Ncbi Gene Id Number:
1901
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
S1P1 Antibody: Movement of lymphocytes through lymphoid organs is required for generating immunity. Their migration into lymph nodes follows a series of events including integrin activation through chemokine signaling, adhesion and diapedis. The release of lymphocytes from lymph nodes is regulated by the phospholipid sphingosine-1-phosphate (S1P). One of its receptors S1P1 binds S1P with high specificity and affinity; agonism of this receptor by the immunosuppressive agent FTY720 inhibits the entry of tissue T cells into afferent lymphatics in homeostatic and inflammatory conditions. Recent experiments have indicated that CCR7-deficient T cells left lymph nodes more rapidly than wild-type cells did and these cells where also less effectively retained after treatment with FTY720, and that egress competence could be restored by inactivating G alpha i-protein-coupled receptor signaling. These results suggest that S1P1 acts in the lymphocyte to promote lymph node egress by overcoming retention signals mediated by CCR7 and G alpha i-protein-coupled receptor signaling.
Background Reference 1:
Miyasaka M and Tanaka T. Lymphocyte tracking across high endothelial venules: dogmas and enigmas. Nat. Rev. Immunol.2004; 3:360-70.
Background Reference 2:
Mandala S, Hajdu R, Bergstrom J, et al. Alteration of lymphocyte tracking by sphingosine-1-phosphate receptor agonists. Science 2002; 296:346-9.
Background Reference 3:
Ledgerwood LG, Lal G, Zhang N, et al. The sphingosine-1-phosphate receptor 1 causes tissue retention by inhibiting the entry of peripheral tissue T lymphocytes into afferent lymphatics. Nat. Immunol. 2008; 9:42-53.
Background Reference 4:
Pham TH, Okada T, Matloubian M, et al. S1P1 receptor signaling overrides retention mediated by G alpha i-coupled receptors to promote T cell egress. Immunity 2008; 28:122-33.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4809

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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