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Western blot analysis of S1PR2 in HeLa cell lysate with S1PR2 antibody at 1 μg/ml.
Immunohistochemistry of S1PR2 in mouse brain tissue with S1PR2 antibody at 10 μg/ml.
Immunofluorescence of S1PR2 in mouse brain tissue with S1PR2 antibody at 20 μg/ml.<br><br>Red: S1PR2 Antibody (8229) <br> Blue: DAPI staining
Western blot analysis of S1PR2 in HeLa cell lysate with S1PR2 antibody at 1 μg/ml.
Trial Size

S1PR2 Antibody

CATALOG NUMBER: 8229

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
S1PR2 antibody was raised against an 16 amino acid peptide near the carboxy terminus of human S1PR2.
The immunogen is located within amino acids 270 - 320 of S1PR2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
S1PR2 antibody can be used for detection of S1PR2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry at 10 μg/ml.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
S1PR2 antibody is human, mouse and rat reactive.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Predicted Molecular Weight:
Predicted: 39 kDa
Observed: 50 kDa
Purification:
S1PR2 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
S1PR2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
S1PR2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
S1PR2
Additional Names:
Sphingosine 1-phosphate receptor 2, S1P2, AGR16, EDG-5, EDG5, Gpcr13, H218, LPB2
Protein Accession Number:
NP_004221
Protein Gi Number:
134244587
Ncbi Gene Id Number:
9294
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The Sphingosine 1-phosphate receptor 2 (S1PR2) protein was initially identified as G-protein coupled receptor thought to be involved in development (1) and was found to mediate sphingosine 1-phosphate (SPP)-induced cell rounding and neurite retraction (2). S1PR2 and the related protein S1PR3 (also known as EDG3) are also thought to mediate the SPP-induced cell proliferation, survival, and related signaling events (3). S1PR2 has also been found to be key regulator in acute vascular inflammation and may be a novel therapeutic target for vascular disorders (4).
Background Reference 1:
MacLennan AJ, Browe CS, Gaskin AA, et al. Cloning and characterization of a putative G-protein coupled receptor potentially involved in development. Mol. Cell Neurosci. 1994; 5:201-9.
Background Reference 2:
Van Brocklyn JR, Tu Z, Edsall LC, et al. Sphingosine 1-phosphate-induced cell rounding and neurite retraction are mediated by the G protein-coupled receptor H218. J. Biol. Chem. 1999; 274:4626-32.
Background Reference 3:
An S, Zheng Y, and Bleu T. Sphingosine 1-phosphate-induced cell proliferation, survival, and related signaling events mediated by G protein-coupled receptors Edg3 and Edg5. J. Biol. Chem. 275:288-96.
Background Reference 4:
Zhang G, Yang L, Kim GS, et al. Critical role of sphingosine-1-phosphate receptor 2 (S1PR2) in acute vascular inflammation. Blood 2013; 122:443-55.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8229

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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