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Western blot analysis of lysed extracts from PC12 cells untreated or treated with anisomycin using S6 Ribosomal Protein (Phospho-Ser235).
Immunofluorescence staining of methanol-fixed HeLa cells using S6 Ribosomal Protein (Phospho-Ser235).
Western blot analysis of lysed extracts from PC12 cells untreated or treated with anisomycin using S6 Ribosomal Protein (Phospho-Ser235).

S6 Ribosomal Protein (phospho Ser235) Antibody

CATALOG NUMBER: 79-478

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
S6 Ribosomal Protein (Phospho-Ser235) antibody was raised against a peptide sequence around phosphorylation site of serine 235 (R-L-S (p) -S-L) derived from Human S6 Ribosomal Protein.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of S6 Ribosomal protein only when phosphorylated at serine 235.
Predicted Molecular Weight:
32 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
RPS6
Additional Names:
S6, Mtapt, Tau, NP33, RPS6, RS6
Protein Accession Number:
NP_001001.2
Protein Gi Number:
17158044
Ncbi Gene Id Number:
6194
Background:
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Background Reference 1:
McBride K, et al. (1998) Mol Cell Biol 18 (9): 5073-5081. Williams AJ, et al. (2003) Plant Physiol 132 (4): 2086-2097. Wilson MA, et al. (1997) Biochem J 325 (Pt 1): 217-222. Arnesen T, et al. (2005) Biochem J 386 (Pt 3): 433-443.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-478

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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