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Western blot analysis in 293 cell line lysates (35ug/lane).
SACA1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in 293 cell line lysates (35ug/lane).

SACA1 Antibody

CATALOG NUMBER: 55-410

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SACA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-74 amino acids from the N-terminal region of human SACA1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
32 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SPACA1
Additional Names:
Sperm acrosome membrane-associated protein 1, Sperm acrosomal membrane-associated protein 32, SPACA1, SAMP32
Protein Accession Number:
Q9HBV2
Protein Gi Number:
74752784
Ncbi Gene Id Number:
81833
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The correlation of anti-sperm antibodies with cases ofunexplained infertility implicates a role for these antibodies inblocking fertilization. Improved diagnosis and treatment ofimmunologic infertility, as well as identification of proteins fortargeted contraception, are dependent on the identification andcharacterization of relevant sperm antigens. The protein expressedby this gene is recognized by anti-sperm antibodies from infertilemales. Furthermore, antibodies generated against the recombinantprotein block in vitro fertilization. This protein localizes to theacrosomal membrane of spermatids and mature spermatozoa where it isthought to play a role in acrosomal morphogenesis and in sperm-eggbinding and fusion, respectively.
Background Reference 1:
Yokoyama, K., et al. Nephron Clin Pract 115 (4), C237-C243 (2010) :
Background Reference 2:
Levy, D., et al. BMC Med. Genet.SUPPL 1, S3 (2007) :
Background Reference 3:
Vasan, R.S., et al. BMC Med. Genet.SUPPL 1, S2 (2007) :
Background Reference 4:
Mungall, A.J., et al. Nature 425(6960):805-811(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-410

List Size:
400 ul

List Price:

$495.00

Datasheet
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