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Western blot analysis of SAE2 in 293 cell lysate with SAE2 antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of SAE2 in 293 cells with SAE2 antibody at 4 μg/mL.
Immunofluorescence of SAE2 in 293 cells with SAE2 antibody at 20 μg/mL.
Western blot analysis of SAE2 in 293 cell lysate with SAE2 antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

SAE2 Antibody

CATALOG NUMBER: 5751

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (94%)
Immunogen:
SAE2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SAE2.
The immunogen is located within amino acids 550 - 600 of SAE2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
SAE2 antibody can be used for detection of SAE2 by Western blot at 0.25 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 17-010 - HEK293 Cell Slide
Purification:
SAE2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SAE2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SAE2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UBA2
Additional Names:
SAE2 Antibody: ARX, SAE2, HRIHFB2115, UBLE1B, SUMO-activating enzyme subunit 2, Anthracycline-associated resistance ARX
Protein Accession Number:
NP_005490
Protein Gi Number:
4885649
Ncbi Gene Id Number:
10054
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SAE2 Antibody: Small ubiquitin-like modifiers (SUMOs) are a family of small, related proteins (SUMO-1/2/3/4) that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation, a major regulator of protein function in cellular processes such as nuclear transport, transcriptional regulation, apoptosis and protein stability. This sumoylation is effected by the heterodimeric enzyme SAE1/SAE2 and the SUMO-1-conjugating enzyme Ubch9. The sumoylation pathway mediated by SAE1/SAE2 is distinct from other ubiquitin-like protein (Ubl) pathways.
Background Reference 1:
Kamitani T, Kito K, Nguyen HP, et al. Characterization of a second member of the sentrin family of ubiquitin-like proteins. J. Biol. Chem.1998;273:11349-53.
Background Reference 2:
Kim KI, Baek SH, and Chung CH. Versatile protein tag, SUMO: its enzymology and biological function. J. Cell. Physiol.2002; 191: 257-68.
Background Reference 3:
Desterro JM, Rodriguez MS, Kemp GD, et al. Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1. J. Biol. Chem.1999; 274:10618-24.
Background Reference 4:
Tatham MH, Jaffray E, Vaughan OA, et al. Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9. J. Biol. Chem.2001; 276:35368-74.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5751

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

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