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SALL1 Antibody

CATALOG NUMBER: 42-194

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse
Immunogen:
The immunogen for this antibody is: EKGQPSRPTKSKDAH-C
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Blot: Preliminary experiments gave an approx 100kDa band in Human Brain (Amygdala and Substantia nigra) and Mouse Kidney lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 140kDa according to Human NP_002959.2 and Mouse NP_067365.2, and 130kDa according to Human NP_001121364.1. The 100kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificity:
This antibody is expected to recognize both reported isoforms (NP_002959.2; NP_001121364.1).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SALL1
Additional Names:
SALL1, sal-like 1 (Drosophila), HSAL1, TBS, ZNF794, sal-like 1, spalt-like transcription factor 1
Protein Accession Number:
NP_002959.2,NP_001121364.1
Protein Gi Number:
118442837
Ncbi Gene Id Number:
6299
Background Reference 1:
Barry JS, Reddy MA The association of an epibulbar dermoid and Duane syndrome in a patient with a SALL1 mutation (Townes-Brocks Syndrome). Ophthalmic Genet. 2008 Dec;29(4):177-80.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-194

List Size:
0.1 mg

List Price:

$468.00

Datasheet
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