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Western blot analysis of extracts of various cell lines, using SALL4 Antibody (22-720) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunofluorescence analysis of A549 cells using SALL4 antibody (22-720). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using SALL4 Antibody (22-720) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.

SALL4 Antibody

CATALOG NUMBER: 22-720

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SALL4 (NP_065169.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:100
Positive Control 1:
Jurkat
Positive Control 2:
HeLa
Positive Control 3:
Mouse heart
Predicted Molecular Weight:
Observed: 80kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SALL4
Additional Names:
SALL4, DRRS, DJ1112F19.1, HSAL4, Sal-like 4 (Drosophila), Sal-like protein 4, Zinc finger protein SALL4, ZNF797, Sal (Drosophila)-like 4, Zinc finger protein 797
Ncbi Gene Id Number:
57167
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a zinc finger transcription factor thought to play a role in the development of abducens motor neurons. Defects in this gene are a cause of Duane-radial ray syndrome (DRRS). Alternative splicing results in multiple transcript variants encoding different isoforms.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-720

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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