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Western blot analysis in Y79,A2058,CEM cell line lysates(35ug/lane)
Western blot analysis in Y79,A2058,CEM cell line lysates(35ug/lane)

SAP Antibody

CATALOG NUMBER: 62-882

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This SAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-314 amino acids from the Central region of human SAP.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
58 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PSAP
Additional Names:
Prosaposin, Proactivator polypeptide, Saposin-A, Protein A, Saposin-B-Val, Saposin-B, Cerebroside sulfate activator, CSAct, Dispersin, Sphingolipid activator protein 1, SAP-1, Sulfatide/GM1 activator, Saposin-C, A1 activator, Co-beta-glucosidase, Glucosylceramidase activator, Sphingolipid activator protein 2, SAP-2, Saposin-D, Component C, Protein C, PSAP, GLBA, SAP1
Protein Accession Number:
P07602
Protein Gi Number:
134218
Ncbi Gene Id Number:
5660
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PSAP is a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities.
Background Reference 1:
Gunia,S., Virchows Arch. 454 (5), 573-579 (2009)
Background Reference 2:
Kuchar,L., Am. J. Med. Genet.149A (4), 613-621 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-882

List Size:
400 ul

List Price:

$495.00

Datasheet
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