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Western blot analysis of SAPAP1 in rat brain tissue lysate with SAPAP1 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of SAPAP1 in human brain with SAPAP1 antibody at 5 μg/mL.
Immunofluorescence of SAPAP1 in Human Brain cells with SAPAP1 antibody at 20 μg/mL.
Western blot analysis of SAPAP1 in rat brain tissue lysate with SAPAP1 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

SAPAP1 Antibody

CATALOG NUMBER: 4623

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SAPAP1 antibody was raised against a 18 amino acid synthetic peptide from near the center of human SAPAP1.
The immunogen is located within amino acids 640 - 690 of SAPAP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SAPAP1 antibody can be used for detection of SAPAP1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of SAPAP1 are known to exist. This SAPAP1 antibody will not cross-react with other SAPAP proteins.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
SAPAP1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SAPAP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SAPAP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DLGAP1
Additional Names:
SAPAP1 Antibody: DAP1; GKAP; DAP-1; SAPAP1; DLGAP1A; DLGAP1B; DAP-1-BETA; DAP-1-ALPHA.
Protein Accession Number:
NP_004737
Protein Gi Number:
4758822
Ncbi Gene Id Number:
9229
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SAPAP1 Antibody: SAP90/PSD-95-associated protein 1 (SAPAP1, also known as DLGAP1 or GKAP) is a member of a protein family whose members specifically interact with PSD-95/SAP90, a membrane-associated guanylate kinase localized at postsynaptic density (PSD) in neuronal cells. Like the other SAPAP proteins, SAPAP1 is thought to be an adaptor protein that also interacts with different synaptic scaffolding proteins, cytoskeletal and signaling components, such as focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2). SAPAP1 mRNA is targeted to cell bodies in a similar manner to SAPAP2 and -4, whereas SAPAP3 mRNA is detected mainly in cell bodies. SAPAP1 protein however, is targeted to the synapse and is not reliant on the synaptic localization of PSD-95 or the synaptic scaffolding molecule (S-SCAM).
Background Reference 1:
SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density. J. Biol. Chem.1997; 272:11943-51.
Background Reference 2:
Kindler S, Rehbein M, Classen B, et al. Distinct spatiotemporal expression of SAPAP transcripts in the developing rat brain: a novel dendritically localized mRNA. Brain Res. Mol. Brain Res.2004; 126:14-21.
Background Reference 3:
Bongiorno-Borbone L, Kadare G, Benfenati F, et al. FAK and PYK2 interact with SAP/PSD-95-associated protein-3. Biochem. Biophys. Res. Commun.2005; 337:641-6.
Background Reference 4:
Welch JM, Wang D, and Feng G. Differential mRNA expression and protein localization of the SAP90/PSD-95-associated proteins (SAPAPs) in the nervous system of the mouse. J. Comp. Neurol.2004; 472:24-39.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4623

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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