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Western blot analysis of SAPAP2 in Raji cell lysate with SAPAP2 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of SAPAP2 in human brain with SAPAP2 antibody at 5 μg/mL.
Immunofluorescence of SAPAP2 in Human Brain cells with SAPAP2 antibody at 20 μg/mL.
Western blot analysis of SAPAP2 in Raji cell lysate with SAPAP2 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

SAPAP2 Antibody

CATALOG NUMBER: 4625

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SAPAP2 antibody was raised against a 16 amino acid synthetic peptide from near the center of human SAPAP2.
The immunogen is located within amino acids 480 - 530 of SAPAP2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SAPAP2 antibody can be used for detection of SAPAP2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of SAPAP2 are known to exist. This SAPAP2 antibody will not cross-react with other SAPAP proteins.
Positive Control 1:
Cat. No. 1207 - Raji Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 43 kDa
Observed: 52 kDa
Purification:
SAPAP2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SAPAP2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SAPAP2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DLGAP2
Additional Names:
SAPAP2 Antibody: DAP2, SAPAP2, DAP2, Disks large-associated protein 2, DAP-2
Protein Accession Number:
Q9P1A6
Protein Gi Number:
160395585
Ncbi Gene Id Number:
9228
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SAPAP2 Antibody: SAP90/PSD-95-associated protein 2 (SAPAP2, also known as DLGAP2) is a member of a protein family whose members specifically interact with PSD-95/SAP90, a membrane-associated guanylate kinase localized at postsynaptic density (PSD) in neuronal cells. Like the other SAPAP proteins, SAPAP2 is thought to be an adaptor protein that also interacts with different synaptic scaffolding proteins, cytoskeletal and signaling components, such as focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2). SAPAP2 mRNA is targeted to cell bodies in a similar manner to SAPAP1 and -4, whereas SAPAP3 mRNA is detected mainly in cell bodies.
Background Reference 1:
SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density. J. Biol. Chem.1997; 272:11943-51.
Background Reference 2:
Ranta S, Zhang Y, Ross B, et al. Positional cloning and characterization of the human DLGAP2 gene and its exclusion in progressive epilepsy with mental retardation. Eur. J. Hum. Genet.2000; 8:381-4.
Background Reference 3:
Kindler S, Rehbein M, Classen B, et al. Distinct spatiotemporal expression of SAPAP transcripts in the developing rat brain: a novel dendritically localized mRNA. Brain Res. Mol. Brain Res.2004; 126:14-21.
Background Reference 4:
Bongiorno-Borbone L, Kadare G, Benfenati F, et al. FAK and PYK2 interact with SAP/PSD-95-associated protein-3. Biochem. Biophys. Res. Commun.2005; 337:641-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4625

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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