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SAR1B antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MCF-7,MDA-MB453 cell line lysates (35ug/lane).
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
SAR1B antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

SAR1B Antibody

CATALOG NUMBER: 55-163

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Hamster, Mouse, Pig, Rat
Immunogen:
This SAR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-116 amino acids from the Central region of human SAR1B.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:50~100
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
22 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SAR1B
Additional Names:
GTP-binding protein SAR1b, GTP-binding protein B, GTBPB, SAR1B, SARA2, SARB
Protein Accession Number:
Q9Y6B6
Protein Gi Number:
14285769
Ncbi Gene Id Number:
51128
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a small GTPase thatacts as a homodimer. The encoded protein is activated by theguanine nucleotide exchange factor PREB and is involved in proteintransport from the endoplasmic reticulum to the Golgi. This proteinis part of the COPII coat complex. Defects in this gene are a causeof chylomicron retention disease (CMRD), also known as Andersondisease (ANDD). Two transcript variants encoding the same proteinhave been found for this gene.
Background Reference 1:
Cefalu, A.B., et al. Metab. Clin. Exp. 59(4):463-467(2010)
Background Reference 2:
Silvain, M., et al. Clin. Genet. 74(6):546-552(2008)
Background Reference 3:
Charcosset, M., et al. Mol. Genet. Metab. 93(1):74-84(2008)
Background Reference 4:
Jardim, D.L., et al. J. Biochem. Mol. Biol. 38(3):328-333(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-163

List Size:
400 ul

List Price:

$495.00

Datasheet
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