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<strong>Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) 3L-PRO in COVID-19 Patient Lung</strong><br> Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) 3L-PRO (NSP5) antibody (9153, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 3L-PRO (NSP5) protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
<strong>Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Protein </strong><br>Antibodies: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody, 9153. A direct ELISA was performed using SARS-CoV-2 3CL-PRO (NSP5) recombinant protein (10-400) as coating antigen and the anti-SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL
<strong>Figure 3 Western Blot Validation with SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Protein</strong><br>Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) recombinant protein (10-400).Antibodies: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5), 9153, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL
Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) 3L-PRO in COVID-19 Patient Lung
Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) 3L-PRO (NSP5) antibody (9153, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 3L-PRO (NSP5) protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
New
Trial Size

SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody

CATALOG NUMBER: 9153

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Virus
Homology:
Predicted reactivity based on immunogen sequence: SARS-CoV 3CL-PRO (NSP5) protein: (100%)
Immunogen:
Anti-SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody (9153) was raised against a peptide corresponding to 16 amino acids near the amino terminus of SARS-CoV-2 (COVID-19) 3CL-Pro (NSP5) protein.
The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) 3CL-Pro (NSP5) protein.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
IHC: 0.5 μg/mL; WB: 0.5 μg/mL
Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. It can detect SARS-CoV-2 NSP5 recombinant protein by ELISA and WB. All other applications and species not yet tested.
Purification:
SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody can be stored at 4 ° C for three months and -20 ° C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
3CL-PRO
Additional Names:
3C-like proteinase, 3CLp, nsp5, Non-structure protein 5, Main protease, Mpro
Protein Accession Number:
YP_009742612.1
Protein Gi Number:
1826688922
Ncbi Gene Id Number:
43740578
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. 3C- like proteinase (NSP5) cleaves the C-terminus of replicase polyprotein at 11 sites (3,4,5). It recognizes substrates containing the core sequence [ILMVF]-Q-, -[SGACN]. It also able to bind an ADP-ribose-1''-phosphate (ADRP) (5).
Background Reference 1:
Gorbalenya. bioRxiv: 2020.
Background Reference 2:
Hui et al. Int J Infect Dis. 2020;91:264-266.
Background Reference 3:
Zhang et al. Science. 2020; 0:0-0(2020)
Background Reference 4:
Jin et al. Nature, 0:0-0(2020)
Background Reference 5:
Dai et al. Science 0:0-0(2020)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

9153

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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