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<strong>Figure 1 ELISA Binding Assay of ACE2 and SARS-CoV-2 (COVID-19) Delta Variant Spike S1 Recombinant Protein </strong><br>Coating Antigen: SARS-CoV-2 Delta variant (B.1.671.2) spike S1 protein, 1 μg/mL, incubated at 4 °C overnight.Detection Antibodies: ACE2 recombinant protein, 10-120, dilution: 0.3- 1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-human HRP at 1:10,000, incubate at RT for 1 hr.
<strong>Figure 2 SDS-PAGE Validation of SARS-CoV-2 (COVID-19) Delta Variant Spike S1 Recombinant Protein </strong><br>Loading: 1 μg SARS-CoV-2 (COVID-19) Delta variant Spike S1 recombinant protein, 95-127.Observed: 120kD
<strong>Figure 3 ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Delta Variant Spike Antibodies </strong><br>Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 °C overnight.Detection Antibodies: SARS-CoV-2 Delta variant spike 156-157EFdel antibody, 9689, and spike 156-157EF antibody, 9685, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.<strong>SARS-CoV-2 Delta Variant Spike S1 protein (95-127), but not WT spike S1 protein (10-300), was specifically detected by Delta Variant spike antibody (9689), while the corresponding WT antibody (9685) detected spike S1 protein of WT, but not delta variant. </strong>
<strong>Figure 4 ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Delta Variant Spike Antibodies </strong><br>Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 °C overnight.Detection Antibodies: SARS-CoV-2 Delta variant spike P681R antibodies, PM-9677 and PM9680, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.<strong>SARS-CoV-2 Delta Variant Spike S1 protein (95-127), but not WT spike S1 protein (10-300), was specifically detected by Delta Variant spike antibodies. </strong>
<strong>Figure 5 WB Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Delta Variant Spike Antibodies </strong><br>Loading: 20 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2).Detection Antibodies: SARS-CoV-2 Delta variant spike antibody, 9689, 1 μg/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.<strong>SARS-CoV-2 Delta Variant S1 protein (95-127), but not WT spike S1 protein (10-300), was specifically detected by Delta Variant spike antibody (9689). </strong>
<strong>Figure 6 ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Spike S1 Antibodies </strong><br>Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 °C overnight.Detection Antibodies: SARS-CoV-2 Spike S1 antibody, 9083, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.<strong>Antibody 9083 does not distinguish between WT and Delta. </strong>
Figure 1 ELISA Binding Assay of ACE2 and SARS-CoV-2 (COVID-19) Delta Variant Spike S1 Recombinant Protein
Coating Antigen: SARS-CoV-2 Delta variant (B.1.671.2) spike S1 protein, 1 μg/mL, incubated at 4 °C overnight.Detection Antibodies: ACE2 recombinant protein, 10-120, dilution: 0.3- 1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-human HRP at 1:10,000, incubate at RT for 1 hr.

SARS-CoV-2 (COVID-19) Delta Variant Spike S1 (His-Avi Tag) Recombinant Protein

CATALOG NUMBER: 95-127

Tested Applications:
ELISA, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Protein
source-species:
HEK293 cells
Recombinant Protein Sequence:
Val 16 - Arg 685 (T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R)-His-Avi
Fusion Tag:
His-Avi tag at C-terminus
Tested Applications:
ELISA, SDS-PAGE, WB
Application Note:
This recombinant protein can be used for WB and ELISA. For research use only.
Predicted Molecular Weight:
120 kD
Purity:
> 90% as determined by Bis-Tris PAGE
physical-state:
Liquid
Buffer:
PBS (pH 7.4).
Storage Conditions:
Store in working aliquots at -70°C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Ncbi Official Symbol:
S
Additional Names:
S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD, Delta variant spike RBD
Protein Accession Number:
QHD43416.1
Ncbi Gene Id Number:
43740578
Background:
SARS-CoV-2 delta variant, a variant of concern (VOC), known as B.1.617.2, was detected in India in October of 2020. However, it rapidly spread all over of the world and now it is the dominant variant in the world, which account for more than 99% of the cases. This variant carries at least 13 mutations in spike protein across the sub lineages, including L452R, D614G, P681R and K417N, which can increase the affinity to the human ACE2 receptor. Enhanced transmission of the Delta variant was observed globally, which is at least 2.5 times more contagious as the other variants. The Delta variant affects the effectiveness of COVID19 vaccine and is resistant to neutralization to some extent.
Background Reference 1:
Zhang et al. JAMA 2021, 325 (13): 1324-26.
Background Reference 2:
Reardon. Nature 2021.
Background Reference 3:
Delphine. Nature 2021, 596 (7871): 276-280.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

95-127

List Size:
0.05 mg

List Price:

$289.00

Datasheet
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